Elaborate separation techniques are necessary to remove toxic substances from the plant extracts under investigation. In some cases, potentially toxic compounds may only have a healing effect if applied in the right dose. Then, precisely adjusted and consistent distribution of the active substances in the final product has to be ensured by refined extraction and enrichment procedures.
Separation of herbal multi-component extracts
Plants normally contain hundreds or thousands of small, low molecular compounds, so called metabolites, in varying concentrations. Prior to the mass spectrometric analyses of plant extracts, the individual components of the samples have to be separated by UHPLC (Ultra High Performance Liquid Chromatography). Our Analytical Department has the specialized knowledge and skill required for the separation of complex mixtures of substances based on the Institute of Analytical Chemistry and Radiochemistry of the University Innsbruck. Additionally, the ADSI GmbH disposes of extensive machinery for extraction and analytics, comprising among others 4 HPLC devices, which are partially directly connected to the mass spectrometers.
ADSI GmbH – High-End Techniques in Plant Screening
High Content Screening applied instead of standard High Throughput Screening adds to quality rather than quantity. Several parameters can be read simultaneously, which allows for a highly differentiated biologic interpretation.
ASAP (AUTOMATED SCREENING ASSAY PLATFORM)
ASAP is a unique fully automated system for carrying out cell-based assays, developed by ADSI to facilitate the required speed and throughput for High Content Screening. By means of ASAP the cell cultures are automatically maintained, treated with test substances, and subsequently analyzed by confocal fluorescence microscopy. In addition, hundreds of cytokines and other molecules involved in cell communication can be measured simultaneously using multiplexing methods (e.g. Luminex technology, etc.). In this manner, it is possible to measure whether single cells or cell groups are permanently damaged by a test substance or whether their communication with each other is impaired. The aim is to obtain as much information as possible about the effect of the tested substances. Data acquired by all these methods allows for a comprehensive analysis and interpretation of complex biological systems.
The amount of data generated by High Content Screening is huge. For their analysis we apply highly advanced bioinformatics and biostatistics.